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How to autoclave glassware
time2011/10/18
Autoclaves are used to sterilize materials. An autoclave uses steam and reaches 121 degrees Celsius and 15 pounds per square inch to effectively kill microorganisms that may be present on the materials. Autoclaves are used commonly in the scientific and medical fields, but they can also be used to sterilize materials needed for brewing beer or canning at home. If used properly, an autoclave will prevent mold and other types of bacteria from growing on the materials.
Put on the latex or nitrile gloves. It is important to avoid touching the inside of the glassware with bare hands. The autoclave can't remove oils transferred from your hands to the glass.Tear the aluminum foil into a section 2 to 4 inches bigger than the opening of the glassware. Avoid overhandling the aluminum foil to minimize the chances of contamination.Cover the opening of the glassware with the flat sheet of aluminum foil. Create a lid by pushing the edges of the aluminum foil down and molding the foil to the shape of the glassware. Assure that there are no holes in the aluminum foil and that it extends at least 1 inch down the outside of the container.
Place the glassware on your heat-resistant tray. If you are autoclaving more than one piece of glassware, do not overload the tray with glassware, or else all items may not reach the necessary temperature.Insert the tray into the autoclave and close the autoclave securely. Autoclaves often have wheels that must be turned to lock the doors shut.
Put on the latex or nitrile gloves. It is important to avoid touching the inside of the glassware with bare hands. The autoclave can't remove oils transferred from your hands to the glass.Tear the aluminum foil into a section 2 to 4 inches bigger than the opening of the glassware. Avoid overhandling the aluminum foil to minimize the chances of contamination.Cover the opening of the glassware with the flat sheet of aluminum foil. Create a lid by pushing the edges of the aluminum foil down and molding the foil to the shape of the glassware. Assure that there are no holes in the aluminum foil and that it extends at least 1 inch down the outside of the container.
Place the glassware on your heat-resistant tray. If you are autoclaving more than one piece of glassware, do not overload the tray with glassware, or else all items may not reach the necessary temperature.Insert the tray into the autoclave and close the autoclave securely. Autoclaves often have wheels that must be turned to lock the doors shut.